About Custom Polyclonal Antibody

Polyclonal antibodies are generated from multiple B cells that recognize distinct epitopes on a single antigen. This allows for more robust detection and greater overall antibody affinity. Polyclonal antibodies are easy to produce and can be ready to use in under four months. They can also bind to multiple epitopes, providing more robust detection than monoclonal antibodies. They are ideal as the capture antibody in a Sandwich ELISA.

Antibodies are critical Custom Polyclonal Antibody reagents that require the immunization of a living animal host to produce. This can be difficult to scale up as it requires a number of animals for adequate antibody production. In addition, the use of animal-based antibodies can be costly and a source of potential ethical concerns.

Monoclonal Antibody Production: Our Process Explained

Therefore, researchers often prefer to use polyclonal antibodies to avoid these ethical issues and meet their research goals. Custom polyclonal antibody production services are available to provide researchers with highly specific and cost-effective antibodies. These services utilize a variety of host species including rabbits, goats, guinea pigs and chickens. These animals are preferred for antibody generation because they are larger, allowing for a higher amount of serum collection with minimal injections. Moreover, they are more easily handled, facilitating more humane injection methods.

The antibodies produced by the animal hosts are collected from the animal’s blood and purified through a series of steps. Antibody titer analysis is then used to determine the amount of antibodies that are present in the serum. The titer of an antibody reflects its ability to bind and recognize the target protein. The titer of an antibody can be used to predict the performance of an antibody in a particular application, and it is critical for quality control procedures. This is why it is important to use a CRO with experience in antibody production under GMP standards.